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post hoc neurobiotin reconstruction  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology post hoc neurobiotin reconstruction
    Figure 4. Whole-cell recordings of LRMGP-EPSCs in pPC AAV-ChR2-injected mice. (A) Representative experiment with AAV-ChR2 injection in the pPC; a, confocal image of 300-lm thick horizontal slice showing the pPC injection site (white arrow) and the recording sites (red boxes); b, <t>neurobiotin-labeled</t> neurons; c, monosynaptic AMPA (downward) and AMPA + NMDA (upward) current traces recorded from neurons in three individual cortices. Note the differences of scale bars. (B, C) Comparisons of normalized monosynaptic AMPA and NMDA current strength. (D) a, Bright-field image of an exemplar pair of IN and PYR recorded in LEC with neurobiotin labeling; b-d, three summary plots showing the comparisons of AMPA, NMDA, and their ratios, respectively, between INs and nearby PYRs recorded in aPC and LEC for pPC injection experiments. *P < 0.05, **P < 0.01.
    Post Hoc Neurobiotin Reconstruction, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2441 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/post+hoc+neurobiotin+reconstruction/goat+anti-rabbit+IgG-HRP+Cruz+Marker+compatible/pm26416972-66-22-30
    Average 96 stars, based on 2441 article reviews
    post hoc neurobiotin reconstruction - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Hierarchical organization of long-range circuits in the olfactory cortices."

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices.

    Journal: Physiological reports

    doi: 10.14814/phy2.12550

    Figure 4. Whole-cell recordings of LRMGP-EPSCs in pPC AAV-ChR2-injected mice. (A) Representative experiment with AAV-ChR2 injection in the pPC; a, confocal image of 300-lm thick horizontal slice showing the pPC injection site (white arrow) and the recording sites (red boxes); b, neurobiotin-labeled neurons; c, monosynaptic AMPA (downward) and AMPA + NMDA (upward) current traces recorded from neurons in three individual cortices. Note the differences of scale bars. (B, C) Comparisons of normalized monosynaptic AMPA and NMDA current strength. (D) a, Bright-field image of an exemplar pair of IN and PYR recorded in LEC with neurobiotin labeling; b-d, three summary plots showing the comparisons of AMPA, NMDA, and their ratios, respectively, between INs and nearby PYRs recorded in aPC and LEC for pPC injection experiments. *P < 0.05, **P < 0.01.
    Figure Legend Snippet: Figure 4. Whole-cell recordings of LRMGP-EPSCs in pPC AAV-ChR2-injected mice. (A) Representative experiment with AAV-ChR2 injection in the pPC; a, confocal image of 300-lm thick horizontal slice showing the pPC injection site (white arrow) and the recording sites (red boxes); b, neurobiotin-labeled neurons; c, monosynaptic AMPA (downward) and AMPA + NMDA (upward) current traces recorded from neurons in three individual cortices. Note the differences of scale bars. (B, C) Comparisons of normalized monosynaptic AMPA and NMDA current strength. (D) a, Bright-field image of an exemplar pair of IN and PYR recorded in LEC with neurobiotin labeling; b-d, three summary plots showing the comparisons of AMPA, NMDA, and their ratios, respectively, between INs and nearby PYRs recorded in aPC and LEC for pPC injection experiments. *P < 0.05, **P < 0.01.

    Techniques Used: Injection, Labeling

    Figure 5. Whole-cell recordings of LRMGP-EPSCs in LEC AAV-ChR2-injected mice. (A) Representative experiment with AAV-ChR2 injection in the LEC; a, bright-field image of 300-lm thick horizontal slice showing the LEC injection site (white arrow) and the recording sites (red boxes). The fluorescence of the recorded slice was quenched after DAB staining for neurobiotin, but an adjacent slice in the inset without staining shows that the injection site is located exactly in LEC; b, neurobiotin-labeled neurons; c, monosynaptic AMPA (downward) and AMPA + NMDA (upward) current traces recorded from neurons in three individual cortices. Note the differences of scale bars. (B, C) Comparisons of normalized monosynaptic AMPA and NMDA current strength. (D) a, Bright-field image of an exemplar pair of IN and PYR; b-d, three summary plots showing the comparisons of AMPA, NMDA, and their ratios, respectively, between INs and nearby PYRs recorded in aPC and pPC for LEC injection experiments. *P < 0.05, **P < 0.01.
    Figure Legend Snippet: Figure 5. Whole-cell recordings of LRMGP-EPSCs in LEC AAV-ChR2-injected mice. (A) Representative experiment with AAV-ChR2 injection in the LEC; a, bright-field image of 300-lm thick horizontal slice showing the LEC injection site (white arrow) and the recording sites (red boxes). The fluorescence of the recorded slice was quenched after DAB staining for neurobiotin, but an adjacent slice in the inset without staining shows that the injection site is located exactly in LEC; b, neurobiotin-labeled neurons; c, monosynaptic AMPA (downward) and AMPA + NMDA (upward) current traces recorded from neurons in three individual cortices. Note the differences of scale bars. (B, C) Comparisons of normalized monosynaptic AMPA and NMDA current strength. (D) a, Bright-field image of an exemplar pair of IN and PYR; b-d, three summary plots showing the comparisons of AMPA, NMDA, and their ratios, respectively, between INs and nearby PYRs recorded in aPC and pPC for LEC injection experiments. *P < 0.05, **P < 0.01.

    Techniques Used: Injection, Staining, Labeling

    Related Articles

    Injection:

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices.
    Article Snippet: Whole-cell recordings were obtained from either nonGABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67GFP fluorescent imaging.Whole-cell recordings were obtained from either nonGABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67GFP fluorescent imaging.. Recorded neurons were typically located 50–100 lm below the surface of the recorded slice and had relatively intact dendritic arbors, verified by post hoc neurobiotin reconstruction (antibodies used from the Santa Cruz Biotechnology, Dallas, TX: goat anti-rabbit HRP, sc-2030; Rb HRP, sc-2749; donkey anti-goat HRP, sc2056).. The holding potential was set at 70 mV to get the putative AMPA (a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.The holding potential was set at 70 mV to get the putative AMPA (a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices
    Article Snippet: Whole-cell recordings were obtained from either non-GABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67-GFP fluorescent imaging.Whole-cell recordings were obtained from either non-GABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67-GFP fluorescent imaging.. Recorded neurons were typically located 50–100 μ m below the surface of the recorded slice and had relatively intact dendritic arbors, verified by post hoc neurobiotin reconstruction (antibodies used from the Santa Cruz Biotechnology, Dallas, TX: goat anti-rabbit HRP, sc-2030; Rb HRP, sc-2749; donkey anti-goat HRP, sc2056).. The holding potential was set at –70 mV to get the putative AMPA ( α -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.The holding potential was set at –70 mV to get the putative AMPA ( α -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.

    Labeling:

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices.
    Article Snippet: Whole-cell recordings were obtained from either nonGABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67GFP fluorescent imaging.Whole-cell recordings were obtained from either nonGABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67GFP fluorescent imaging.. Recorded neurons were typically located 50–100 lm below the surface of the recorded slice and had relatively intact dendritic arbors, verified by post hoc neurobiotin reconstruction (antibodies used from the Santa Cruz Biotechnology, Dallas, TX: goat anti-rabbit HRP, sc-2030; Rb HRP, sc-2749; donkey anti-goat HRP, sc2056).. The holding potential was set at 70 mV to get the putative AMPA (a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.The holding potential was set at 70 mV to get the putative AMPA (a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices
    Article Snippet: Whole-cell recordings were obtained from either non-GABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67-GFP fluorescent imaging.Whole-cell recordings were obtained from either non-GABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67-GFP fluorescent imaging.. Recorded neurons were typically located 50–100 μ m below the surface of the recorded slice and had relatively intact dendritic arbors, verified by post hoc neurobiotin reconstruction (antibodies used from the Santa Cruz Biotechnology, Dallas, TX: goat anti-rabbit HRP, sc-2030; Rb HRP, sc-2749; donkey anti-goat HRP, sc2056).. The holding potential was set at –70 mV to get the putative AMPA ( α -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.The holding potential was set at –70 mV to get the putative AMPA ( α -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.

    Staining:

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices.
    Article Snippet: Whole-cell recordings were obtained from either nonGABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67GFP fluorescent imaging.Whole-cell recordings were obtained from either nonGABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67GFP fluorescent imaging.. Recorded neurons were typically located 50–100 lm below the surface of the recorded slice and had relatively intact dendritic arbors, verified by post hoc neurobiotin reconstruction (antibodies used from the Santa Cruz Biotechnology, Dallas, TX: goat anti-rabbit HRP, sc-2030; Rb HRP, sc-2749; donkey anti-goat HRP, sc2056).. The holding potential was set at 70 mV to get the putative AMPA (a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.The holding potential was set at 70 mV to get the putative AMPA (a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices
    Article Snippet: Whole-cell recordings were obtained from either non-GABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67-GFP fluorescent imaging.Whole-cell recordings were obtained from either non-GABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67-GFP fluorescent imaging.. Recorded neurons were typically located 50–100 μ m below the surface of the recorded slice and had relatively intact dendritic arbors, verified by post hoc neurobiotin reconstruction (antibodies used from the Santa Cruz Biotechnology, Dallas, TX: goat anti-rabbit HRP, sc-2030; Rb HRP, sc-2749; donkey anti-goat HRP, sc2056).. The holding potential was set at –70 mV to get the putative AMPA ( α -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.The holding potential was set at –70 mV to get the putative AMPA ( α -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.

    Fluorescence:

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices.
    Article Snippet: Whole-cell recordings were obtained from either nonGABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67GFP fluorescent imaging.Whole-cell recordings were obtained from either nonGABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67GFP fluorescent imaging.. Recorded neurons were typically located 50–100 lm below the surface of the recorded slice and had relatively intact dendritic arbors, verified by post hoc neurobiotin reconstruction (antibodies used from the Santa Cruz Biotechnology, Dallas, TX: goat anti-rabbit HRP, sc-2030; Rb HRP, sc-2749; donkey anti-goat HRP, sc2056).. The holding potential was set at 70 mV to get the putative AMPA (a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.The holding potential was set at 70 mV to get the putative AMPA (a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices
    Article Snippet: Whole-cell recordings were obtained from either non-GABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67-GFP fluorescent imaging.Whole-cell recordings were obtained from either non-GABAergic, presumed glutamatergic pyramidal neurons (PYRs, including semilunar cells) or GABAergic interneurons (INs) in aPC, pPC, and LEC with the aid of infrared differential interference contrast imaging and GAD67-GFP fluorescent imaging.. Recorded neurons were typically located 50–100 μ m below the surface of the recorded slice and had relatively intact dendritic arbors, verified by post hoc neurobiotin reconstruction (antibodies used from the Santa Cruz Biotechnology, Dallas, TX: goat anti-rabbit HRP, sc-2030; Rb HRP, sc-2749; donkey anti-goat HRP, sc2056).. The holding potential was set at –70 mV to get the putative AMPA ( α -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.The holding potential was set at –70 mV to get the putative AMPA ( α -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid)-mediated currents.



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    post hoc neurobiotin reconstruction  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology post hoc neurobiotin reconstruction
    Figure 4. Whole-cell recordings of LRMGP-EPSCs in pPC AAV-ChR2-injected mice. (A) Representative experiment with AAV-ChR2 injection in the pPC; a, confocal image of 300-lm thick horizontal slice showing the pPC injection site (white arrow) and the recording sites (red boxes); b, <t>neurobiotin-labeled</t> neurons; c, monosynaptic AMPA (downward) and AMPA + NMDA (upward) current traces recorded from neurons in three individual cortices. Note the differences of scale bars. (B, C) Comparisons of normalized monosynaptic AMPA and NMDA current strength. (D) a, Bright-field image of an exemplar pair of IN and PYR recorded in LEC with neurobiotin labeling; b-d, three summary plots showing the comparisons of AMPA, NMDA, and their ratios, respectively, between INs and nearby PYRs recorded in aPC and LEC for pPC injection experiments. *P < 0.05, **P < 0.01.
    Post Hoc Neurobiotin Reconstruction, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/post+hoc+neurobiotin+reconstruction/goat+anti-rabbit+IgG-HRP+Cruz+Marker+compatible/pm26416972-66-22-30
    Average 96 stars, based on 1 article reviews
    post hoc neurobiotin reconstruction - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

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    Figure 4. Whole-cell recordings of LRMGP-EPSCs in pPC AAV-ChR2-injected mice. (A) Representative experiment with AAV-ChR2 injection in the pPC; a, confocal image of 300-lm thick horizontal slice showing the pPC injection site (white arrow) and the recording sites (red boxes); b, neurobiotin-labeled neurons; c, monosynaptic AMPA (downward) and AMPA + NMDA (upward) current traces recorded from neurons in three individual cortices. Note the differences of scale bars. (B, C) Comparisons of normalized monosynaptic AMPA and NMDA current strength. (D) a, Bright-field image of an exemplar pair of IN and PYR recorded in LEC with neurobiotin labeling; b-d, three summary plots showing the comparisons of AMPA, NMDA, and their ratios, respectively, between INs and nearby PYRs recorded in aPC and LEC for pPC injection experiments. *P < 0.05, **P < 0.01.

    Journal: Physiological reports

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices.

    doi: 10.14814/phy2.12550

    Figure Lengend Snippet: Figure 4. Whole-cell recordings of LRMGP-EPSCs in pPC AAV-ChR2-injected mice. (A) Representative experiment with AAV-ChR2 injection in the pPC; a, confocal image of 300-lm thick horizontal slice showing the pPC injection site (white arrow) and the recording sites (red boxes); b, neurobiotin-labeled neurons; c, monosynaptic AMPA (downward) and AMPA + NMDA (upward) current traces recorded from neurons in three individual cortices. Note the differences of scale bars. (B, C) Comparisons of normalized monosynaptic AMPA and NMDA current strength. (D) a, Bright-field image of an exemplar pair of IN and PYR recorded in LEC with neurobiotin labeling; b-d, three summary plots showing the comparisons of AMPA, NMDA, and their ratios, respectively, between INs and nearby PYRs recorded in aPC and LEC for pPC injection experiments. *P < 0.05, **P < 0.01.

    Article Snippet: Recorded neurons were typically located 50–100 lm below the surface of the recorded slice and had relatively intact dendritic arbors, verified by post hoc neurobiotin reconstruction (antibodies used from the Santa Cruz Biotechnology, Dallas, TX: goat anti-rabbit HRP, sc-2030; Rb HRP, sc-2749; donkey anti-goat HRP, sc2056).

    Techniques: Injection, Labeling

    Figure 5. Whole-cell recordings of LRMGP-EPSCs in LEC AAV-ChR2-injected mice. (A) Representative experiment with AAV-ChR2 injection in the LEC; a, bright-field image of 300-lm thick horizontal slice showing the LEC injection site (white arrow) and the recording sites (red boxes). The fluorescence of the recorded slice was quenched after DAB staining for neurobiotin, but an adjacent slice in the inset without staining shows that the injection site is located exactly in LEC; b, neurobiotin-labeled neurons; c, monosynaptic AMPA (downward) and AMPA + NMDA (upward) current traces recorded from neurons in three individual cortices. Note the differences of scale bars. (B, C) Comparisons of normalized monosynaptic AMPA and NMDA current strength. (D) a, Bright-field image of an exemplar pair of IN and PYR; b-d, three summary plots showing the comparisons of AMPA, NMDA, and their ratios, respectively, between INs and nearby PYRs recorded in aPC and pPC for LEC injection experiments. *P < 0.05, **P < 0.01.

    Journal: Physiological reports

    Article Title: Hierarchical organization of long-range circuits in the olfactory cortices.

    doi: 10.14814/phy2.12550

    Figure Lengend Snippet: Figure 5. Whole-cell recordings of LRMGP-EPSCs in LEC AAV-ChR2-injected mice. (A) Representative experiment with AAV-ChR2 injection in the LEC; a, bright-field image of 300-lm thick horizontal slice showing the LEC injection site (white arrow) and the recording sites (red boxes). The fluorescence of the recorded slice was quenched after DAB staining for neurobiotin, but an adjacent slice in the inset without staining shows that the injection site is located exactly in LEC; b, neurobiotin-labeled neurons; c, monosynaptic AMPA (downward) and AMPA + NMDA (upward) current traces recorded from neurons in three individual cortices. Note the differences of scale bars. (B, C) Comparisons of normalized monosynaptic AMPA and NMDA current strength. (D) a, Bright-field image of an exemplar pair of IN and PYR; b-d, three summary plots showing the comparisons of AMPA, NMDA, and their ratios, respectively, between INs and nearby PYRs recorded in aPC and pPC for LEC injection experiments. *P < 0.05, **P < 0.01.

    Article Snippet: Recorded neurons were typically located 50–100 lm below the surface of the recorded slice and had relatively intact dendritic arbors, verified by post hoc neurobiotin reconstruction (antibodies used from the Santa Cruz Biotechnology, Dallas, TX: goat anti-rabbit HRP, sc-2030; Rb HRP, sc-2749; donkey anti-goat HRP, sc2056).

    Techniques: Injection, Staining, Labeling